TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Relevant characteristic(s) | Source or reference |
|---|---|---|
| E. coli | ||
| WM3064 | Cloning and donor strain for conjugation; ΔdapA | 21 |
| S. piezotolerans | ||
| WP3 | Wild type | Laboratory stock |
| WP3ΔEPA | WP3 Δswp3547 (EPA-deficient mutant) | This study |
| WP3ΔLIV | WP3 Δswp3487 (LIV-I function-deficient mutant) | This study |
| Plasmids | ||
| pRE112 | Allelic-exchange vector | 18 |
| pRE112-EPA | pRE112 containing deletion structure for swp3547; used to make WP3ΔEPA | This study |
| pRE112-LIV | pRE112 containing deletion structure for swp3487; used to make WP3ΔLIV | This study |