TABLE 2.
Specific activities of formate dehydrogenase and hydrogenase in the washed cells of the parental strain and the mopB mutant grown on lactate-sulfate mediuma
| Strain | Sp act (μmol min−1·mg−1) of:
|
|||
|---|---|---|---|---|
| Formate dehydrogenase
|
Hydrogenase
|
|||
| MV | BV | MV | BV | |
| Parent | 0.69 ± 0.19 | 0.35 ± 0.21 | 9.6 ± 0.8 | 9.4 ± 1.8 |
| mopB mutant | 1.49 ± 0.28 | 0.64 ± 0.17 | 10.3 ± 2.0 | 10.0 ± 2.2 |
a
MV or BV was used as the electron acceptor in the assays. Activity was expressed as the mean ± the standard deviation (n = 3) in micromoles of MV or BV per minute per milligram of protein.