TABLE 2.

Immunofluorescent labeling of wild-type and rpoS mutant B. burgdorferi organisms within fed Ixodes nymphs

Antigen	Percentage of antigen-labeled spirochetes in the indicated population of FlaB-labeled spirochetesa		Pb
	Wild type	rpoS mutant
BBA74	74.13 ± 6.43	91.18 ± 5.48	0.002
OspA	77.33 ± 2.91	94.22 ± 3.11	0.004
OspE	53.90 ± 4.94	51.27 ± 72.12	NS
OspC	49.43 ± 2.25	0.6 ± 1.07	0.005

^aA minimum of 1,000 FlaB-labeled organisms were counted for each double-labeling experiment. Values presented are the average of three biologically independent experiments ± standard deviations.

^bNS, not significant.