TABLE 3.
Infectivity of isolates B31-A3 and B31-MI
| Isolate and dose (no. of spirochetes/ mouse [ID50])a | No. of culture-positive samples/ total no. of samplesb
|
|||
|---|---|---|---|---|
| Ear | Joint | Bladder | All sites | |
| B31-A3 (890 ± 155) | ||||
| 104 | 3/3 | 2/3 | 3/3 | 8/9 |
| 103 | 2/3 | 2/3 | 2/3 | 6/9 |
| 102 | 0/3 | 0/3 | 0/3 | 0/9 |
| B31-MI (1,450 ± 150) | ||||
| 104 | 3/3 | 3/3 | 2/3 | 8/9 |
| 103 | 1/3 | 1/3 | 1/3 | 3/9 |
| 102 | 0/3 | 0/3 | 0/3 | 0/9 |
a
C3H/HeJ mice (three per group) were needle inoculated with each isolate at the dosage indicated. B31-A3 harbors a truncated BBA74 protein while isolate B31-MI expresses the full-length protein. ID50 values were calculated using an algorithm provided by the National Center for Biotechnology Information.
b
Based on the presence of spirochetes 3 to 5 weeks following culture in BSK medium.