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. 2009 Feb 18;83(9):4678–4689. doi: 10.1128/JVI.02401-08

FIG. 2.

FIG. 2.

Time course of JcDNV delivery to early and late endocytic compartments: colocalization with endocytic markers. (A) Cells were concomitantly infected with JcDNV and either TRITC-Tfn, FITC-albumin, or LysoTracker red for 30 min or 4 h at 28°C. After fixation cells were labeled with anti-JcDNV antibody (green or red) and endocytic markers (red or green), and nuclei were labeled with DAPI (blue). Scale bar, 20 μm. (B). Time-dependent colocalization of JcDNV with Tfn, albumin, or LysoTracker. The percentage of colocalized JcDNV with Tfn (diamond), albumin (square), or LysoTracker (triangle) over three consecutive stacks (0.3 μm) is shown. Colocalization was significant, with a Pearson coefficient of >0.9.