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. 2009 Feb 18;83(9):4216–4226. doi: 10.1128/JVI.02436-08

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FIG. 3. — Differential cytoskeleton requirements for intracellular sorting of viruses. (A) Effects of nocodazole on VSV, SVDV, and FMDV infection studied by plaque assay. (B) Evaluation of nocodazole treatment on microtubule and Golgi integrity. Cells treated or not with nocodazole were fixed and processed for immunofluorescence using rabbit polyclonal antiserum against tubulin and MAb 25H8 against cis-Golgi protein gp74. Anti-rabbit Alexa Fluor 488-coupled and anti-mouse Alexa Fluor 594-coupled secondary antibodies were used. Bar, 20 μm. (C) Effects of Cyt D on virus infection studied by plaque assay. (D) Evaluation of Cyt D treatment on actin cytoskeleton integrity. Cells treated or not with Cyt D were fixed and processed for immunofluorescence using Alexa Fluor 488-labeled phalloidin to stain the cellular actin. Bar, 20 μm. Statistically significant differences between control and drug treatments are indicated by one asterisk (P < 0.05) or two asterisks (P < 0.005).