FIG. 5.
The putative leucine zipper of HSV-1(F) pUL6 is required for interaction with pUL28 in the absence of other viral proteins. Cells were transfected with plasmids encoding full-length pUL28 and wild-type pUL6 (lane 1), pUL6 lacking codons 422 to 443 (lane 2), or pUL6 in which codons 422 to 443 were replaced by a leucine zipper of GCN4 (lane 3). The cells were lysed 24 h later and either immunoblotted with antibodies directed against pUL6 or pUL28 (A and B) or subjected to immunoprecipitation with antibody directed against pUL28. The presence of pUL6 or pUL28 in immunoprecipitated material was determined by immunoblotting (C and D, respectively). IP, antibody used for immunoprecipitation in the corresponding panel; IB, antibody used for immunoblotting in the corresponding panel. The arrows indicate the positions of proteins of interest.