TABLE 1.
Virus replication assay
| Virus | Genotype of UL6 | Cell type infected/cell type used for plaque assay | Titer (PFU/ml)a |
|---|---|---|---|
| vJB10 | Deletion of codons 422 to 443 | CV1/CV6 | <102 |
| vJB10 | Deletion of codons 422 to 443 | CV6/CV6 | 1.2 × 107 ± 0.5 × 107 |
| vJB10 | Deletion of codons 422 to 443 | CV6M/CV6 | <102 |
| vJB10R | Deleted codons restored | CV1/Vero | 2.0 × 107 ± 0.76 × 107 |
| HSV-1(F) | Wild type | CV1/Vero | 2.5 × 107 ± 0.2 × 107 |
| HSV-1(F) | Wild type | CV6/Vero | 2.6 × 107 ± 0.5 × 107 |
| HSV-1(F) | Wild type | CV6M/Vero | 6.5 × 104 ± 0.8 × 104 |
a
Virus titers were determined as described in Materials and Methods. The data represent means ± standard deviations for three independent experiments.