FIG. 7.

Both R5 HIV and X4 HIV bind equally to naïve and memory resting CD4⁺ T cells. Sorted naïve (CD45RA⁺ CD62L⁺) and memory (CD45RA⁻ and/or CD62L⁻) resting CD4⁺ T cells were spinoculated with BaL (R5 HIV) and pNL4-3 (X4 HIV) for 2 h at 25°C. The level of viral binding was estimated by measuring the level of cell-associated p24^Gag by ELISA immediately after inoculation and after removal of unbound virions. The graph represents the averages of a total of six measurements of viral binding from three independent inoculations. Error bars represent the standard errors. The P values were calculated with the Wilcoxon rank-sum test.