FIG. 2.

Expression and stability of RLuc-E2 fusion proteins measured using luciferase activity. (A) Luciferase activity measured in C33A cells transfected with increasing amounts of RLuc-E2 vectors or RLuc control vector. For each measurement, the levels of RLuc activity were normalized to those of FLuc expressed from a cotransfected plasmid. (B) The stabilities of RLuc proteins were determined 48 h posttransfection by measuring the levels of luciferase activity remaining at different time points (in minutes) after a block of protein synthesis with 100 μg of cycloheximide/ml.