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. 2009 Feb 11;83(9):4476–4488. doi: 10.1128/JVI.02110-08

FIG. 10.

FIG. 10.

Susceptibility to cold inactivation of HIV-1 envelope glycoproteins in the CD4-bound conformation. (A) The infectivities of viruses with the HXBc2, HXBc2-H66N, and HXBc2-S375W envelope glycoproteins after incubation on ice for the indicated times were measured. The relative infectivity represents the percentage of the observed luciferase counts relative to those seen with freshly thawed virus. (B) Relative infectivities of viruses with the wild-type HXBc2 envelope glycoproteins following incubation on ice with the indicated concentrations of NBD-556. (C) Relative infectivities of viruses with the HXBc2, HXBc2-H66N, and HXBc2-S375W envelope glycoproteins after incubation on ice in the presence of 2 μM NBD-556. (D) Infectivities of viruses with the wild-type HXBc2 envelope glycoproteins following incubation on ice or at room temperature (r.t.) for 50 h in the presence of the indicated concentrations of NBD-556. (E) Viruses with the wild-type HXBc2 envelope glycoproteins were incubated on ice in the absence or presence of sCD4 (0.1 μg/ml), and the infectivities were measured. (F) Relative infectivities of viruses with the YU2 envelope glycoproteins following incubation on ice in the absence (control) or presence of 100 μM NBD-556 or 1 μg/ml sCD4. The means and standard deviations from three independent experiments performed in triplicate are shown.