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Binding of gp120 ligands to cell surface HIV-1 envelope glycoproteins after incubation on ice. Mock-transfected 293T cells and 293T cells expressing cytoplasmic tail-deleted ADA-N197S, ADA-H66N-N197S, KB9, or KB9-H66N envelope glycoproteins were incubated on ice or at room temperature for 16 h. Binding of the indicated gp120 ligands was assessed by FACS. The percentages of antibody-positive cells are shown.
