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Effect of cold incubation on the CD4-induced exposure of the gp41 HR1 region. Mock-transfected 293T cells and 293T cells expressing cytoplasmic-tail-deleted ADA-N197S, ADA-H66N-N197S, KB9, or KB9-H66N envelope glycoproteins were incubated on ice or at room temperature for 16 h. The binding of C34-Ig and 2G12, after incubation with the indicated ligands, was assessed by FACS.
