FIG. 8.
Exposure of HIV-1 envelope glycoprotein epitopes on virions. Viruses with VSV G and ADAfl, KB9, or YU2 envelope glycoproteins or their H66N counterparts were kept at −80°C (initial) or incubated on ice or at room temperature for 50 h. Control viruses with only VSV G (VSV) or lacking envelope glycoproteins [ENV(−)VSV(−)] were treated in parallel. Viruses were captured on wells coated with the indicated antibodies. After a washing, Cf2Th cells lacking HIV-1 receptors were added to the wells, and luciferase activity was assessed 48 h later. The results of a single experiment, performed in triplicate, are shown. The experiment was repeated twice with similar results.