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Oct-2 represses the binding of Oct-1 and ORF50 to the ORF50 promoter. (A) ChIP assay results show the binding of Oct-1, Oct-2, and ORF50 to the endogenous ORF50 promoter in BC1/Tet-on/Oct-2 cells under specified conditions. A control IgG corresponding to each IP antibody was used to verify specificity. Data shown are representative of three independent experiments. (B) Expression of Oct-1, Oct-2, and ORF50 under the various conditions was confirmed by Western blotting.
