FIG. 1.

Effects of endocytosis inhibitors on HSV-1 entry into cells. (A) CHO-hPILRα and CHO-hNectin-1 cells were infected with YK333 at an MOI of 1 at 4°C for 1 h. The inoculum was then removed, and cells were treated with glucose-free medium containing 2% bovine serum albumin, 0.3% 2-deoxy-d-glucose, and 0.05% sodium azide and held at 4°C for 15 min and then at 37°C for 30 min. Virus that had not penetrated the cells was inactivated by acid treatment, and infection continued for an additional 7 h, after which infected cells were analyzed by flow cytometry. The relative mean fluorescence intensity (MFI) of infected cells treated with energy depletion medium was calculated as the percentage relative to that of mock-treated infected cells. The mean and standard deviation from three independent experiments is shown for each cell type. (B) CHO-hPILRα or CHO-hNectin-1 cells were pretreated with the indicated concentrations of monensin for 1 h at 37°C. Cells were then infected with YK333 at an MOI of 1 for 7 h in the presence of the drug and analyzed by flow cytometry. The relative MFI of infected cells was calculated as the percentage relative to that in the absence of the drug. The mean and standard deviation from three independent experiments is shown for each data point for each cell type.