FIG. 2.

NF-κB is a repressor of K-Rta. (A) 293 cells were cotransfected with K-Rta, K-Rta-responsive promoter, and NF-kB (RelA). Luciferase activity of K-Rta alone was normalized to a value of 1. (B) Effects of canonical and noncanonical pathways of NF-κB on K-Rta activity. 293 cells were cotransfected with different combinations of NF-κB proteins, K-Rta, and ORF57 promoter. (C) Enhancement of K-Rta activity by NF-κB inhibitor. 293 cells were cotransfected with K-Rta, ORF57 promoter, and the indicated plasmid. RelA significantly inhibits K-Rta transactivation, and IκBα enhances K-Rta activity. RLU, relative light units.