TABLE 1.
Primers used for cloning
| Primer | Sequence (5′→3′)a |
|---|---|
| RBP-Jκ CpoI, F | aaaCGGTCCGATGGGGGGCTGCAGGAAATTTGGTGA |
| RBP-Jκ CpoI, R | aaaCGGACCGTTAGGATACCACTGTGGCTGTAG |
| NF-κB1 CpoI, F | aaaCGGTCCGATGGCAGAAGATGATCCATATTTG |
| NF-κB1 433 CpoI, R | aaaCGGACCGTTACATGGTTCCATGCTTCATCCCAG |
| NF-κB2 CpoI, F | aaaCGGTCCGATGGAGAGTTGCTACAACCCAGGT |
| NF-κB2 473 CpoI, R | aaaCGGACCGTTACAGCGCGCGCGCGTCCGCGGTGA |
| RelA CpoI, F | aaaCGGTCCGATGGACGATCTGTTTCCCCTCAT |
| RelA CpoI, R | aaaCGGACCGTTAGGAGCTGATCTGACTCAAA |
| RelB CpoI, F | aaaCGGTCCGATGCCGAGTCGCCGCGCTGCCAG |
| RelB CpoI, R | aaaCGGACCGCTACGTGGCTTCAGGCCCTGGA |
| c-Rel CpoI, F | aaaCGGTCCGATGGCCTCCGGTGCGTATAACCCGTA |
| c-Rel CpoI, R | aaaCGGACCGTTATACTTGAAAAAATTCATATGGAA |
| IκBα CpoI, F | aaaCGGTCCGATGTTCCAGGCGGCCGAGCG |
| IκBα CpoI, R | aaaCGGACCGTCATAACGTCAGACGCTGGCCTC |
a
Underlined nucleotides represent restriction enzyme (CpoI) sites used for cloning the PCR products; lowercase letters indicate nucleotides added to enhance recognition by the restriction enzyme. F, forward; R, reverse.