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. 2009 Feb 18;83(9):4287–4296. doi: 10.1128/JVI.02399-08

FIG. 5.

FIG. 5.

Detection of viral RNA in BALFs of infected pigs using real-time RT-PCR. Copy numbers in log10 per 1 ml of BALFs obtained by real-time RT-PCR targeting the matrix protein gene are given for individual animals. The white columns represent piglets inoculated with the 1930/rec influenza virus at 3 dpi (pigs 66, 67, and 83) 5 dpi (pigs 69, 71, 84, and 85), and at 7 dpi (pigs 86 and 87). The gray columns represent piglets inoculated with the 1918/rec virus at 3 dpi (pigs 88, 89, 97, 98, and 103) and at 5 dpi (pigs 90, 91, 99, 100, and 104). Black diamonds indicate samples where no viral RNA was detected: at 7 dpi, pigs 92, 93, 101, 102, and 105 inoculated with the 1918/rec virus and piglets 70 and 72 inoculated with the 1930/rec virus; at 3 dpi, pig 82 inoculated with the 1930/rec virus. Viral RNA was not detected in the 1918/rec virus-inoculated piglets after 7 dpi.