TABLE 1.
E1B-55K mutant proteins and their known phenotypesa
| E1B-55K proteinb | Protein stability | Substrate degradation
|
Substrate binding
|
E4orf6 binding | E1B-AP5 binding | Nuclear localization | Aggresome formation | Inhibition of viral genome concatemers | mRNA export and host cell shut-off | Late protein synthesis | Replication efficiency | Primary rodent cell transformation | Reference(s) | |||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| p53 | MRN complex | Ligase IV | p53 | MRN complex | ||||||||||||
| wt | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | |
| H17in | + | ND | ND | ND | + | ND | + | + | ND | ND | ND | ± | ± | − | − | 44, 77, 126, 155 |
| L83/L87/L89A | + | ± | ± | + | + | + | ND | ND | ++ | − | ND | + | + | + | ++ | 37, 79, 132 |
| K104R | + | + | ± | ND | ND | + | ND | ND | + | + | ND | + | + | + | − | 35, 79 |
| NES/K104R | + | + | ± | ND | ND | + | ND | ND | + | + | ND | + | + | + | ND | 79 |
| A143in | + | ND | ND | ND | + | ND | − | + | ++ | ND | ND | ± | ± | ± | ++ | 44, 50, 77, 126, 155 |
| H180in | ± | ND | ND | ND | − | − | − | − | − | − | ND | − | − | − | ± | 44, 50, 77, 93, 126, 155 |
| H215in | − | ND | ND | ND | ND | ND | ND | ND | − | ND | ND | − | − | ± | − | 50, 155 |
| H224in | + | + | + | + | + | + | ± | ± | ± | + | + | ± | ± | ± | ± | 44, 50, 77, 126, 132, 155 |
| R240A | + | − | + | + | − | + | + | ND | + | + | + | + | + | + | ND | 22, 86, 132, 134 |
| H260A | + | − | ND | ND | − | ND | − | ND | − | ND | ND | ± | + | ± | ND | 115, 134 |
| A262in | ± | − | ND | ND | − | − | − | − | − | − | ND | − | − | − | − | 44, 50, 77, 93, 119, 126, 155 |
| R309in | ± | − | ND | ND | − | − | − | − | − | − | ND | − | − | − | − | 44, 50, 77, 93, 119, 126, 155 |
| H326in | ± | − | ND | ND | − | − | − | − | − | − | ND | ± | ± | − | ± | 44, 50, 77, 93, 126, 136, 155 |
| H354in | + | + | − | + | + | − | + | ± | + | + | + | ± | ± | ± | ± | 22, 44, 50, 77, 86, 93, 126, 155 |
| C361/C366S | ± | − | − | − | ± | − | ND | ND | ± | + | − | ND | ND | ND | ND | 132 |
| H373A | + | + | ± | + | + | − | ND | ND | + | + | + | ND | ND | ND | ND | 132 |
| S380in | + | + | + | ND | ± | ND | + | − | − | + | ND | + | + | + | ± | 44, 50, 77, 93, 126, 155 |
| R443in | + | ± | − | ± | + | − | + | + | ± | ± | − | ± | ± | − | − | 35, 44, 50, 77, 126, 132, 136, 155 |
| Y444A | + | + | ± | + | + | ± | ND | ND | ± | + | + | ND | + | ± | ND | 132, 134 |
| C454/C456S | ± | − | − | − | + | − | ND | ND | ± | + | − | ND | ND | ND | − | 69, 132 |
| H474in | − | ND | ND | ND | ND | ND | ND | ND | − | ND | ND | − | − | − | ± | 50, 155 |
| F484in | ± | ND | ND | ND | ± | ND | + | − | − | ND | ND | ± | ± | ± | + | 44, 50, 77, 126, 155 |
| S490/S491A | + | + | ± | + | + | ND | + | + | + | ND | ND | + | ± | − | − | 44, 119, 126, 132, 140 |
| S490/S491/T495A | + | − | − | − | − | − | + | ND | ± | − | − | ND | ND | ND | − | 69, 119, 132, 141 |
| S490/S491/T495D | + | ± | + | + | + | + | ND | ND | + | + | + | ND | ND | ND | ND | 132 |
a
List of E1B-55K mutants and their known properties in viral infection and cell transformation. Abbreviations: +, proficient; ±, partially defective; −, severely defective; ND, not determined.
b
The “in” suffix indicates an insertion mutant.