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. 2009 Feb 25;83(9):4345–4353. doi: 10.1128/JVI.02195-08

FIG. 1.

FIG. 1.

Knockdown of BPLF1 mRNA levels decreases the EBV genome copy number. 293EBV+ cells were induced by transfection with Z (BZLF1) along with shRNA against BPLF1. 893S and 3650S denote scrambled forms of 893 and 3650 shRNA constructs. Z serves as the positive control and pcDNA3 as the negative control. (A) RT-PCR of RNA from 293EBV+ cells. Seventy-two hours after transfection, cells were lysed and total RNA was extracted and subjected to RT-PCR using primers against the N-terminal or C-terminal portions of BPLF1. (B) Real-time PCR of 4-μl supernatant fluids from shRNA-transfected 293EBV+ cells at 72 h. Genomic DNA was extracted from supernatant fluids and analyzed by real-time PCR with a TaqMan probe corresponding to the BamHI sequence. The total genome copies present in the positive control (Z) were set to 100%, and samples are reported as a percentage of Z.