Table 1.
Induction of dauer recovery by muscarinic agonists
| Drug | daf-7(e1372) | daf-8(e1377) | daf-14(m77) | daf-2(e1370) | age-1(m333) | age-1(mg44) |
|---|---|---|---|---|---|---|
| No drug/no food | 1.3 ± 1.3 | 4.5 ± 0.1 | 0 | 0 | 0 | 0 |
| Pilocarpine | 8.8 ± 6.0 | 17.7 ± 0.4 | 2.5 ± 0.1 | 0 | ND | ND |
| Arecoline | 34.3 ± 24.4 | 34.0 ± 0.7 | 0.2 ± <0.1 | 0 | 0 | 0 |
| Oxotremorine | 41.2 ± 24.5 | 36.2 ± 0.3 | 14.3 ± <0.1 | 0 | 0 | 0 |
| Oxotremorine atropine | 5.2 ± 2.7 | 4.8 ± 0.01 | <0.1 ± <0.1 | 0 | ND | ND |
Experiments were performed as in Materials and Methods. Animals were scored 24–48 h after being placed as dauer larvae on plates containing drugs. Values are mean ± SE. Numbers shown indicate the percent dauer recovery from two trials, where each plate was at least in duplicate for daf-7(e1372), daf-2(e1370), and age-1(m333). The remaining strains show values from one experiment in which each drug was tested on at least two plates, although similar trends have been observed previously. For each drug tested the number of animals are in the following order: no drug no food; 1 mM pilocarpine; 1 mM arecoline; 1 mM oxotremorine; and 1 mM oxotremorine with 1 mM atropine. Number of animals tested are: daf-7(e1372), 2345, 692, 756, 1363, 1227; daf-8(e1377), 1582, 354, 652, 753, 925; daf-14(m77), 1300, 676, 698, 902, 1101; daf-2(e1370), 872, 222, 258, 207, 460; age-1(m333), 113, not done (ND), 74, 74, ND; age-1(mg44), 71, ND, 57, 52, ND.