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. Author manuscript; available in PMC: 2010 Apr 1.
Published in final edited form as: Cell Metab. 2009 Apr;9(4):327–338. doi: 10.1016/j.cmet.2009.02.006

Figure 3. SIRT1 regulates ligand-dependent PPARα transactivation.

Figure 3

(A) Reduction of SIRT1 activity decreases ligand dependent PPARα transactivation. (Left panel) pSuper (black bars) or pSuper-SIRT1 RNAi (white bars) HEK293T cells were cotransfected with luciferase reporters and a construct encoding Gal4 DBD-PPARα LBD fusion protein, then treated with DMSO or PPARα agonists and analyzed for luciferase activity as described in Experimental Procedures. (Right panel) pSuper (black bars) and pSuper-SIRT1 RNAi (white bars) HEK293T cells were transfected with 3xPPRE-luciferase construct or a control construct lacking the PPRE, together with constructs expressing murine PPARα and RXRα. Cells were then treated with increasing doses of WY14643 and analyzed for luciferase activity as described in the Experimental Procedures. (*p<0.05)

(B) Loss of SIRT1 in primary hepatocytes reduces ligand dependent PPARα transactivation. (*p<0.05)

(C) Overexpression of SIRT1 stimulates ligand-dependent PPARα transactivation in mouse H2.35 hepatoma cells. (*p<0.05)