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. Author manuscript; available in PMC: 2009 May 1.
Published in final edited form as: J Membr Biol. 2008 Jun 25;223(1):13–26. doi: 10.1007/s00232-008-9107-7

Figure 2. nAChR ion channel function and stability assays for lipid-analog detergents.

Figure 2

Planar bilayer current traces at −70 mV membrane potential in the presence of 0.5 μM Carb. (left panels) for: (a) Sodium cholate, (c) FC-12 and (e) CHAPS. A-SEC stability assay percent (%) peak areas (right panels) for sucrose gradient detergent-solubilized membranes (white background) and ligand affinity-purified nAChR (black background) in (b) Sodium cholate (sucrose gradient % peak areas: 65% dimer/35% monomer; affinity column % peak areas: 13% aggregate/58% dimer/29% monomer) (d) FC-12 (sucrose gradient % peak areas: 58% dimer/42% monomer; affinity column % peak areas: 7% aggregate/61% dimer/32% monomer) and (f) CHAPS (sucrose gradient % peak areas: 75% dimer/25% monomer; affinity column % peak areas: 15% aggregate/55% dimer/31% monomer).