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. Author manuscript; available in PMC: 2009 May 1.
Published in final edited form as: J Membr Biol. 2008 Jun 25;223(1):13–26. doi: 10.1007/s00232-008-9107-7

Figure 3. nAChR ion channel function and stability assays for detergents retaining residual amounts of native lipid.

Figure 3

Planar bilayer current traces−70 mV membrane potential in the presence of 0.5 μM Carb. (left panels) for: (a) Anapoe-C12E9 and (c) BigCHAP and A-SEC stability assay percent (%) peak areas (right panels) for sucrose gradient detergent-solubilized membranes (white background) and ligand affinity-purified nAChR (black background) in (b) Anapoe-C12E9 (sucrose gradient % peak areas: 64% dimer/36% monomer; affinity column % peak areas: 20% aggregate/59% dimer/21% monomer) and (d) BigCHAP (sucrose gradient % peak areas: 82% dimer/18% monomer; affinity column % peak areas: 20% aggregate/66% dimer/14% monomer).