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. Author manuscript; available in PMC: 2009 Apr 13.
Published in final edited form as: Mol Endocrinol. 2007 Aug 30;21(12):2907–2918. doi: 10.1210/me.2007-0293

Figure 3. Snail increases invasiveness of MCF-7 cells through matrigel.

Figure 3

MCF-7 cells infected with control or Snail-adenovirus for 3 days were trypsinized, counted, and 20,000 cells were resuspended in DMEM/F-12 medium lacking FBS and pipetted carefully over a membrane coated with Matrigel in the upper well of a Boyden chamber. The lower chamber contained the same media supplemented with 10% FBS as the chemoattractant. After a period of 24 hours, the membranes were removed and the cells that had invaded through the matrigel to the other side were stained with Diff-Quik and counted under the microscope. Data shown are an average of three biological replicates. A t-distribution analysis was performed on the samples within each set (single asterisk * indicates p-value≤0.05, ** indicates p-value ≤ 0.01). A two-sample t-test comparing Snail to control-treated samples showed a p-value of 0.01.