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. Author manuscript; available in PMC: 2010 Mar 1.
Published in final edited form as: Dev Biol. 2008 Dec 3;327(1):24–33. doi: 10.1016/j.ydbio.2008.11.017

Figure 7.

Figure 7

CD34++CD45+ placental cells have multilineage hematopoietic potential. CD34++CD45low cells sorted from a 19-week placenta were cultured at 7.5 × 103 cells/well in 48-well plates in SDM supplemented with SCF+FL+IL-7+IL-15+GM-CSF+IL-3 (A) or SCF+FL+Epo+GM-CSF+TPO (B). Viable cells (5 × 104) were analyzed after 23 days in culture (see Figure 1 legend). Results are representative of 3 experiments. (C) CD34++CD45low cells sorted from a 12-week placenta were cultured at 5 × 104 cells/well in 48-well plates with MS-5 murine BM stromal cells as feeders. After 3 weeks in culture, cells were detached, washed and stained with the indicated mAbs. The results of an analysis using a gate that contained viable CD45+FITC cells (left) and CD45+PE cells (right) are shown.