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. Author manuscript; available in PMC: 2009 Apr 13.
Published in final edited form as: Nat Med. 2002 May;8(5):473–479. doi: 10.1038/nm0502-473

Fig. 3.

Fig. 3

Activation of PI3K/Akt pathway by corticosteroids. a, Thin layer chromatography blots show concentration-dependent effect of Dex with and without RU or LY, and 17β-estradiol (E2; 100 nM), on GR-associated PI3K activity. Experiments performed 3 times with comparable results. b, Effect of ActD and WM on Dex-stimulated PI3K activity and RU on Dex-induced GR-p85α interaction. Following treatment with Dex (100 nM, 20 min), endothelial-cell lysates were immunoprecipitated with GR antibody and then immunoblotted for GR and p85α. 3 experiments yielded similar results. c, Effect of Dex (time-course) or insulin growth factor (IGF; 50 ng/ml, 5 min), with and without RU and LY (at 20 min time point) on Akt and GSK-3 phosphorylation. Experiments performed at least 3 times with similar results. d, Effects of Dex with and without RU or WM on Akt kinase activity. Values are means ± s.e.m.; n = 3, *, P < 0.05 compared with control.