Table 1.
Optimized electroporation conditions
| Pulse intensity (V/cm) | Pulse duration (ms) | Pulse number | Transfection efficiencya (%) | Cell deathb (%) | |
|---|---|---|---|---|---|
| HL-60c (cDNAd) | 670 | 20 | 1 | 45 | 40−45 |
| HL-60c (siRNAe) | 630 | 7 | 2 | 90 | 5−10 |
| Hippocampal neurons (cDNAd) | 530 | 15 | 1 | ∼ 50 | 50−60 |
| HUVEC (cDNAd) | 530 | 15 | 1 | 65 | 60−70 |
| 3T3-L1c (cDNAd) | 470 | 20 | 1 | 55 | 40−50 |
| RBL-2H3 (cDNAd) | 670 | 15 | 1 | 45 | 40−45 |
a
Number of electroporated live cells/number of total live cells.
b
Determined by comparing the number of live cells between treated versus non-treated samples in which the same amount of cells were pipetted.
c
Differentiated cells.
d
Optimized conditions for eGFP cDNA at a concentration of 25−10 ng/μl in extracellular buffer.
e
Optimized conditions for LMNA siRNA at a concentration of 750−500 nM in extracellular buffer. Data shown for HL-60 cells, hippocampal neurons and HUVECs are averages from n = 8−10 experiments; for 3T3-L1 and RBL-2H3 cells, n = 3.