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. Author manuscript; available in PMC: 2010 May 5.
Published in final edited form as: J Control Release. 2009 Feb 5;135(3):250–258. doi: 10.1016/j.jconrel.2009.01.020

Figure 3.

Figure 3

The binding capacity of conjugated anti-DIG IgG on chitosan and the virus immobilization stability were determined by indirect sandwich ELISA assay. (a) The DIG-AdLacZ immobilized on chitosan was proportional to the concentration of incubated DIG-AdLacZ. (b) The immobilization rate of DIG-modified adenovirus was 100% when the concentration was equal to or less than 109 viral particles per well. Higher virus concentrations led to lower immobilization rates due to a limited substrate area. (c) The distribution of immobilized DIG-AdLacZ on anti-DIG conjugated chitosan surfaces was illustrated by SEM examination. The scale bar in the picture is 1 μm. (d) Adenovirus with and without DIG modification were placed on anti-DIG IgG (solid line) and anti-adenovirus IgG (dashed line) conjugated surfaces, respectively. The released viral particles were detected at different time points to determine retention rates. There were no differences between these two groups and more than 75% of the virus could be stably maintained for two weeks.