Adenovirus, with (solid line) or without (dashed line), DIG modification were placed on antibody conjugated chitosan surfaces to investigate the specificity of the conjugated antibody to adenovirus. (a) On anti-DIG IgG conjugated chitosan surfaces, DIG-modified virus was immobilized by surface antibodies in a dose dependent manner, whereas there was only a slight adsorption of non-modified adenovirus due to nonspecific binding. (b) On anti-adenovirus IgG conjugated chitosan, both adenovirus with and without DIG modification were bound to the surfaces with a similar affinity.