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. 2009 Apr 21;4(4):e5250. doi: 10.1371/journal.pone.0005250

Figure 1. ER fission induced by glutamate or NMDA.

Figure 1

(A) Representative image of a dendrite of a living hippocampal neuron transfected to express cytosolic EGFP and RedER showing normal ER morphology. (B) When exposed to 100 µM glutamate, rapid fission of the ER occurred after 1 min. Note the lack of change in dendritic and dendritic spine morphology in the green channel. (C) Image of proximal dendrite exposed to 100 µM glutamate for 20 min clearly showing the fragmented appearance of the ER in the xy (upper panel) and xz (lower panel) dimensions. (D) Representative image of a dendrite with normal morphology. (E) When exposed to 100 µM NMDA, rapid fission of the ER occurred within 3 min. Note the lack of change in dendritic and dendritic spine morphology in the green channel. Scale bar in all panels: 10 µm.