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. Author manuscript; available in PMC: 2010 Jan 15.
Published in final edited form as: Cancer Res. 2009 Jan 15;69(2):678–686. doi: 10.1158/0008-5472.CAN-08-3980

Figure 5. Immunohistochemistry staining patterns of normal appearing small bowel tissues from ApcMin/+;Tgfbr1+/+ and ApcMin/+; Tgfbr1+/− mice.

Figure 5

(A and B) Normal appearing small intestine stained with pSmad2 shows identical staining pattern throughout the villi of both ApcMin/+;Tgfbr1+/+ mice (A) and ApcMin/+; Tgfbr1+/− mice (B). However, pSmad2 staining within the intestinal crypts of ApcMin/+; Tgfbr1+/− mice (arrow) is reduced when compared with that of their wild type counterparts (arrow).

(C and D) Normal appearing small intestine stained with pSmad3 shows identical staining pattern throughout the villi of both ApcMin/+ ;Tgfbr1+/+ mice (C) and ApcMin/+; Tgfbr1+/− mice (D). However, pSmad3 staining within the intestinal crypts of ApcMin/+; Tgfbr1+/− mice (arrow) is reduced when compared with that of their wild type counterparts (arrow).

(E and F) Levels of proliferating cell nuclear antigen (PCNA) expression were significantly higher in ApcMin/+;Tgfbr1+/− intestinal epithelial crypts (F, 62.2 ± 2.2% of positive staining) (arrow) than in their wild type counterpart (E, 44.4 ± 2.8% positive staining) (arrow) (p = 0.008).