Table 1.

NMR restraints and statistics

NMR constraints	Values
Distance constraints	1851
Intraresidual	911
Sequential	483
Medium-range	196
Long-range	215
Ambiguously assigned	46
Dihedral angle constraints*
φ	79
ψ	79
JHNHα coupling constants	20
Residual dipolar couplings
JHN	66
JNC′	50
JC′Cα	54
RMS deviations for constraints
Distance constraints, Å	0.016 ± 0.002
Dihedral angles, degrees	0.33 ± 0.06
JHNHα coupling constants (Hz)	0.78 ± 0.05
RDCs (Hz)
JHN	1.69 ± 0.09
JNC′	0.34 ± 0.01
JC′Cα	0.95 ± 0.06
RMS deviations from covalent geometry
Bond lengths, Å	0.003 ± 0.0002
Bond angles, degrees	0.45 ± 0.014
Impropers, degree	0.31 ± 0.017
Ramachandran plot statistics (%)†	eTAFH (267-353), HEB (14-26)
Residues in most favored regions	85.0
Residues in additionally allowed regions	12.0
Residues in generously allowed regions	2.1
Residues in disallowed regions	1.3
RMS deviations from average structure, Å	eTAFH (270-345), HEB (16-21)
Backbone atoms	0.77 ± 0.13
Heavy atoms	1.59 ± 0.16

^*Backbone dihedral angles from TALOS prediction.

^†Ramachandran plot statistics from PROCHECK-NMR analysis.