Figure 6.

Effect of 5-μg CRH injection on ANP mRNA in rat heart chambers analyzed by Northern blotting after 30 min and 3 hr. (A) Left atrium. (B) Right atrium. (C) Left ventricle. (D) Right ventricle. Insets show representative mRNA hybridization bands obtained with radioactive ANP cDNA probe and radioactive GAPDH cDNA probe used as an internal control. The ANP mRNA level in individual samples was calculated as a signal ratio of ANP and GAPDH bands scanned by PhosphorImager. The histogram depicts average values obtained from scans of three independent experiments (n = 10; *, P < 0.05).