A) Ba/F3 cells expressing TLR4-Flag (TLR4F), TLR4-GFP (TLR4G), MD2-Flag (MD2F), CD14 and NF-κB luciferase were pre-treated with auranofin (5, 10 μM) or sodium tetrachloroaurate (50 μM) for 1 h and then treated with LPS (50 ng/ml) for 20 mins. Cells were then subjected to immunoprecipitation with anti-mouse GFP antibody and immunoblotted with anti-Flag (upper) or anti-rabbit GFP (lower) antibody. B) The same Ba/F3 cells in Fig. 2A were pre-treated with auranofin (5, 10 μM) or sodium tetrachloroaurate (20, 50 μM) for 1 h and then treated with LPS (5 ng/ml) for an additional 6 hrs. Cell lysates were prepared and luciferase enzyme activities were measured as described in the legend of Fig. 1. Values are mean±SEM (n=3). **, Significantly different from LPS alone, p<0.01. The panels are representative data from more than three independent experiments. Veh, vehicle; Au(I), auranofin; Au(III), sodium tetrachloroaurate dihydrate.