Skip to main content
. Author manuscript; available in PMC: 2009 Apr 14.
Published in final edited form as: J Neurochem. 2009 Feb 13;109(2):551–561. doi: 10.1111/j.1471-4159.2009.05989.x

Figure 3. Western blotting of glutaminase during course of HIV-1 infection.

Figure 3

Human MDM were grown in culture and then infected with HIV-1ADA for 1, 3, 5, 7, or 9 days. A, Whole cell lysates were collected and analyzed by Western blot for glutaminase (KGA and GAC). β-actin was used as a loading control. B-C, Levels of GAC (B) and KGA (C) were normalized as a ratio to β-actin after densimetrical quantification and shown as percentage of control (1 dpi). Open bars represent control MDM and solid bars represent HIV-1-infected MDM. D-E, MDM were infected with HIV-1ADA for 7 days with or without AZT treatment (5 μM). Whole-cell lysates were collected and analyzed by Western blotting for GAC (D). GAC Bands were quantified and expressed as percentage of control (E). Quantification results were shown as average ± SEM in experiments performed with 4 different donors. *, p < 0.05 compared with day-matched control. # indicates p < 0.05 when compared to HIV group.