Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2009 Mar 17;132(4):889–902. doi: 10.1093/brain/awp029

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author (2009). Published by Oxford University Press on behalf of the Guarantors of Brain. All rights reserved. For Permissions, please email: journals.permissions@oxfordjournals.org

PMC Copyright notice

Expression of ObR in the arcuate nucleus of B6 (top), diet-induced obesity (DIO) (middle) and A^vy (bottom) mice shown by double immunostaining with M-18 (red) and CH14104 (green) antibodies. (A) M-18 immunoreactivity was mainly seen in astrocytes (arrows). CH14104 antibody stained mostly neurons (arrow heads). In comparison with the lean B6 control, the diet-induced obesityB6 mice and the A^vy mice on a B6 background showed a greater number of ObR(+) astrocytes seen with the M18 antibody (epifluorescence image, scale bar = 50 µm). (B) Higher magnification images showing that astrocytes can be stained with both M-18 and CH14104 antibodies, the former providing a stronger signal with a crisper background (confocal image, scale bar = 10 µm).

Expression of ObR in the arcuate nucleus of B6 (top), diet-induced obesity (DIO) (middle) and A^vy (bottom) mice shown by double immunostaining with M-18 (red) and CH14104 (green) antibodies. (A) M-18 immunoreactivity was mainly seen in astrocytes (arrows). CH14104 antibody stained mostly neurons (arrow heads). In comparison with the lean B6 control, the diet-induced obesityB6 mice and the A^vy mice on a B6 background showed a greater number of ObR(+) astrocytes seen with the M18 antibody (epifluorescence image, scale bar = 50 µm). (B) Higher magnification images showing that astrocytes can be stained with both M-18 and CH14104 antibodies, the former providing a stronger signal with a crisper background (confocal image, scale bar = 10 µm).