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. Author manuscript; available in PMC: 2009 Apr 14.
Published in final edited form as: Comb Chem High Throughput Screen. 2008 Dec;11(10):817–824. doi: 10.2174/138620708786734244

Fig. (4).

Fig. (4)

Concentration-responses of two known GC inhibitors. IC50 values for CBE and nonyl-DNJ were determined using the “blue” and “red” GC enzyme assays. The substrate concentrations were 800 μM 4MU-β-Glc and 30 μM Res-β-Glc, respectively, and the enzyme concentration was 1.9 nM in both assays. The IC50 value for CBE was 15.4 μM in the “blue” (○) and 13.0 μM in the “red” (●) assay. The IC50 value for nonyl-DNJ was 0.227 μM in the “blue” (□) and 0.132 μM in the “red” (■) assay. The concentration-response curves for each inhibitor were very similar in both assays.