Fig. (5).
Results of the LOPAC library screen using the “red” GC enzyme assay. (a) Scatter plot of the results from the control DMSO plate screen. The signal-to-basal ratio was 4.1-fold with a CV of 5.5% and Z’ factor of 0.78. Scatter plots of the LOPAC library screen at (b) 3.06, (c) 15.3 and (d) 76.7 μM compound concentrations. (e) Concentration-response curves of hits with an IC50 ≤10 μM selected from the LOPAC library screen. 13 active compounds were identified, yielding a hit rate of ~1.0 %.