Fig. 3.

Dgt phenotype after RNAi knockdown of human augmin subunits. (A) Reduced spindle localization of γ-tubulin or MT after knockdowns of human augmin subunits. A hDgt3-knockdown cell is shown as a representative. (B) Quantification of spindle localization of γ-tubulin or MT. The ratio of spindle to centrosome signal intensity after knockdowns of human augmin subunits, which was normalized to control (±SEM), was significantly (P < 0.01) lower than that in the control (luciferase RNAi) (n ≥ 9). (C) Astral MTs (arrowheads) emanated from the centrosome (green) in metaphase-arrested cells depleted of hDgt6. GFP-tubulin was imaged in a living cell, and the region around one spindle pole is presented. (Scale bars, 5 μm.)