Table 5.
Viral loads in maternal tissues and blood following inoculation of pregnant hamsters with SV40 strains with different regulatory region structures
| Animal number | Type of regulatory region | Strain/variant | Days post-inoculation (days post-delivery) | SV40 copies/106 cells |
Blood |
|||
|---|---|---|---|---|---|---|---|---|
| Spleen | Kidney | Lung | WBC (SV40 copies/106 cells) | Plasma (SV40 copies/10 μ1) | ||||
| 1 | Simple | Baylor-1E | 2a | 255 | 1,770 | 869 | NDb | 41 |
| 2 | 2a | 1,590 | 3,270 | 3,020 | 10,400 | 22 | ||
| 3 | 2a | <5 | 360 | 891 | 962,000 | 12 | ||
| 4 | 6 (3) | 11,300 | 8,020 | 609,000 | <5 | <5 | ||
| 5c | 10 (7) | 72 | 559 | <5 | ND | ND | ||
| 6c | SVCPC | 10 (7) | 2,460 | 25,000 | <5 | ND | ND | |
| 7c | 17 (14) | 8,680 | 17,800 | <5 | ND | ND | ||
| 8 | 24 (21) | 3,670 | 32,900 | <5 | ND | ND | ||
| 9c | Complex | Baylor-2Ed | 4 (1) | 233,000 | 789,000 | 442,000 | ND | 580 |
| 10c | 6 (3) | 45,400 | 883,000 | 72,200 | ND | 17 | ||
| 11c | 10 (7) | 17,500 | 34,400 | 1,780 | <5 | 16 | ||
| 12 | 17 (14) | 26,100 | 44,400 | 1,470 | 20,600 | 14 | ||
| 13 | 24 (21) | 409 | 3,890 | <5 | 383,000 | <5 | ||
| 14c | SVPML-1 | 10 (7) | 7,040 | 36,900 | 769 | ND | ND | |
| 15c | 17 (14) | 3,180 | 11,900 | <5 | ND | ND | ||
| 16c | 24 (21) | 249 | 810 | <5 | ND | ND | ||
a
Animals from transplacental transmission experiments.
b
ND, not done.
c
Animals with virus-positive offspring.
d
Two animals inoculated with SV40 strain Baylor-2E and harvested 2 days p.i. were negative for SV40 DNA in all tissues tested; one animal inoculated with SV40 strain SVCPC and harvested at 10 days p.i. was not tested (data not shown).