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pVHL is necessary for inhibition of P1465 hydroxylation and Ser5 phosphorylation by SH in PC12 cells. Exposure to the indicated duration of 1% O2 repressed P1465 hydroxylation and Ser5 phosphorylation in PC12 cells expressing wild-type pVHL, but not in cells with diminished levels of pVHL due to the expression of VHL antisense mRNA. Western blots were probed with the indicated antibodies.
