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. 2009 Apr 15;20(8):2132–2145. doi: 10.1091/mbc.E08-10-1046

Figure 1.

Figure 1.

Nuclear division via double restriction and fission of the NE. (A) A nucleus undergoing mitosis observing An-Gle1-GFP. Arrowhead indicates the nuclear remnant generated by a double NE restriction. (B) A telophase nucleus displaying double NE restrictions (An-Gle1-GFP) around the nuclear remnant (arrowhead) and mitotically dispersed An-Nup49-chRFP located back to daughter nuclei but not the NE of the nuclear remnant. (C) Fixed cell image of two telophase nuclei displaying NE restrictions around the nuclear remnant (arrowhead) as revealed by the location of An-Nup96-mRFP. The mitotically dispersed GFP-Nup98 has returned to the NE of daughter nuclei but not the NE of the nuclear remnant. (D) The nuclear envelope (ER/NE marker An-Erg24-GFP) is shown through mitosis (Video1.mov). NE fission occurs first to the left and then to the right, generating the nuclear remnant (arrowheads). (E) A nucleus of a cell expressing An-Erg24-GFP and H1-mRFP progressing through mitosis. The nuclear remnant (arrowheads) does not contain DNA. (F) A fixed germling with nuclei in slightly different stages of mitotic exit. Although daughter nuclei import NLS-DsRed, the nuclear remnant (arrowhead) does not. Bars, ∼5 μm.