Figure 7.

Mitotic-driven partitioning of the nucleolus without mitotic spindle formation. Time-lapse images, kymographs, and line profiles following the indicated tagged proteins in the presence of benomyl during SIM. (A) NLS reimport occurs in an area distinct (arrowhead) from the old nucleolus (An-Bop1-GFP). As G1 continues, An-Bop1-GFP disassembles from the old nucleolus (kymograph o) and reassembles at the opposite side of the nucleus forming the new nucleolus (n). (B) The nucleolus (An-Fib-chRFP) initially contains the unsegregated NORs (An-Topo I-GFP) but begins to separate from the NORs at 4 min (line profile, red arrow). Subsequently, An-Fib disassembles from the old nucleolus and reassembles back at the unsegregated NORs (Video7.mov). Bars, ∼5 μm.