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Requirement of ERK phosphorylation for Xe-Cdc25A degradation. (A–C) Activated eggs were injected with 2 ng of mRNA encoding the indicated Myc-Xe-Cdc25A constructs, reinjected 2.5 h later with 9 ng of MEK-CA mRNA, and analyzed for Myc-Xe-Cdc25A constructs by immunoblotting. In B, 3A/S36, etc., are reversion mutants of SP:4A (see text). Four independent experiments were performed for A–C, and, for each, a typical result is shown.
