Figure 4.

Some components of Paf1C affect nonsense suppression of [PSI+]. (A) Deletion of PAF1 weakens [PSI+]-mediated nonsense suppression. Deletions of PAF1 were made in the following strains: strong (s) [PSI+], weak (w) [PSI+], [psi−], and SV16. Each deletion mutant and parental wild-type control strain was spotted as described in the legend of Figure 3. (B) Deletion of RTF1 suppresses the GdnHCl sensitivity of SV16 but not the reduction in nonsense suppression of ade1-14. A wild-type (wt) strong [PSI+] strain, SV16, SV16 Δrtf1, and strong [PSI+] Δrtf1 strain were spotted as described in the legend of Figure 3.