Figure 6.

ctr9-C227Y reduces nonsense suppression of ade1-14 in the absence of Sup35p aggregates. SV16 [PSI+] was crossed to [psi−] sup35-Y351C, and the resulting diploid was cured of [PSI+] by growth on media containing 3 mM GdnHCl before sporulation. Fivefold serial dilutions of yeast cultures at equal cell density were spotted on YPD and SD-ade. The top four rows show control strong (s) [PSI+] and [psi−] strains and the [PSI+] SV16 ([PSI+] ctr9*) and [psi−] sup35-Y351C ([psi−] sup35*) parental strains used to generate the diploid. The bottom four rows show a representative tetrad with genotype indicated on the left. Presence of the ctr9-C227Y and sup35-Y351C alleles is designated with an asterisk adjacent to the gene name. Wild-type alleles are designated in capital letters. All progeny are [psi−].