Figure 6.

Relationship of dRrp6 to the spindle assembly checkpoint. (A) Metaphase cells that had been treated with taxol were stained with antibodies to dRrp6 or dRrp40 and compared with α-tubulin and DAPI. Bar, 2 μm. (B) Striking dRrp6 and dRrp40 prometaphase distribution revealed through microtubule stabilization. Note how particles of dRrp40 decorate the microtubules extending to the unaligned chromosome. dRrp40 also “sheaths” the unaligned chromosome, whereas dRrp6 is “upon” the chromosomes. Bar, 2 μm. (C) Disruption of MTs elicits accumulation of dRrp6 and dRrp40 around chromosomes with unattached kinetochores. Bar, 2 μm. (D) Taxol or colchicine treatment of dRrp6-depleted cells does not affect the reduced levels of pH3.