Table 1.
Quantitative distribution analysis of dRrp40 and dRrp6 as a function of mitosis and cell division
| Prophase |
Prometaphase |
Metaphase |
Anaphase |
Telophase |
Cytokinesis |
|||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| dRrp6 (n = 12) | dRrp40 (n = 11) | dRrp6 (n = 22) | dRrp40 (n = 32) | dRrp6 (n = 15) | dRrp40 (n = 13) | dRrp6 (n = 18) | dRrp40 (n = 11) | dRrp6 (n = 7) | dRrp40 (n = 7) | dRrp6 (n = 11) | dRrp40 (n = 10) | |
| Nucleus | 40 | 63 | ||||||||||
| Cytoplasm and centrosomal | 60 | 18 | ||||||||||
| Cytoplasm | N/A | 18 | ||||||||||
| Perichromosomal | 18 | 16 | ||||||||||
| Perichromosomal and spindle | N/A | 84 | 40 | 46 | ||||||||
| Chromosomes | 82 | N/A | 60 | N/A | 100 | N/A | ||||||
| Spindle | N/A | 54 | N/A | N/A | ||||||||
| Spindle remnant | N/A | 18 | ||||||||||
| Spindle remnant and chromosomes | N/A | 82 | ||||||||||
| Nascent nuclei | 29 | N/A | ||||||||||
| Nascent nuclei and midbody | 29 | 29 | ||||||||||
| Cytoplasm and midbody | 42 | 71 | 55 | 30 | ||||||||
| Cytoplasm, centrosomal, and midbody | 45 | 60 | ||||||||||
| Nascent nuclei, centrosomal, and midbody | N/A | 10 | ||||||||||
Values are expressed as percent; N/A, not applicable. Only deconvolved images captured with the Deltavision Restoration system were used in this categorization. Representative images of the localization patterns for these different categories are shown in Supplemental Figure S1.